Fisheries
Elham Abdzadeh; Fatemeh Nazarhaghighi
Articles in Press, Accepted Manuscript, Available Online from 02 December 2013
Abstract
To evaluate the levels of cortisol, glucose and other possible indicators of goldfish ,Carassius auratus, in response to stress, in the first experiment, Bisphenol A (0.5 mg/L), Naphthalene (200 μg/L) and Butachlor (60%) (0.28 µg/L) were added to the aquariums water for two weeks. In the second experiment, ...
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To evaluate the levels of cortisol, glucose and other possible indicators of goldfish ,Carassius auratus, in response to stress, in the first experiment, Bisphenol A (0.5 mg/L), Naphthalene (200 μg/L) and Butachlor (60%) (0.28 µg/L) were added to the aquariums water for two weeks. In the second experiment, intra-peritoneal injections of Bisphenol A, Naphthalene and Butachlor,with dose 50 mg/kg, 50 mg/kg, 20 μL on the body weight of fish were performed. On the fifth day and the end of the experiment (fifteenth day) as well as 48 hours after injection, blood sampling from caudal vein was taken and levels of cortisol, glucose, total protein and blood plasma were measured inorganic phosphorous. The results showed that the cortisol and glucose levels on day 5, there was a significant difference between control and Butachlor treatments (P 0.05). Comparing the results of the analysis of blood plasma in the fifth and fifteenth days of experiment, it was found that levels of cortisol and glucose in the treatment Butachlor had significantly different together within two periods (P
behrrouz heidari; Mina Ahmadpour; Razyeh Amani
Abstract
Abstract The high application of the nanoparticles in industry, health and agriculture increases the possibility of nanoparticles entering the environment, especially the aquatic environment. In the present study, the effects of nano colloidal silver and copper oxide nanoparticles (Cu2ONPs) were investigated ...
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Abstract The high application of the nanoparticles in industry, health and agriculture increases the possibility of nanoparticles entering the environment, especially the aquatic environment. In the present study, the effects of nano colloidal silver and copper oxide nanoparticles (Cu2ONPs) were investigated on the liver and blood plasma of the Caspian kutum (Rutilus kutum) fry by measuring the activity of the ALT, AST, ALP and LDH enzymes. The samples (17.1±3.6 g) were prepared from the Propagation and Reconstruction Center of Dr. Yousoufpour. After exposing the fish to different concentrations of the nano colloidal silver and Cu2ONPs, the blood was collected on the 7th and 21st days of the experiment. At the same time, the liver tissue of the fish was removed and stored in the -70oC until analyzes of the parameters. The results showed changes in the activity of the enzymes in the liver and plasma of the Caspian kutum fry. The most changes of enzymes were observed in the plasma on the 7th day of the experiment. The LDH enzyme had the most changes in the plasma other than the enzymes. On the 21st day, only the plasma LDH had a significant variation. In the liver, the levels of ALT, AST, ALP and LDH enzymes were almost increased during exposure to the nanoparticles. In the liver, as with plasma, the most changes of the enzymes belonged to the LDH. In conclusion, fluctuations in the metabolic enzymes indicate a change in the physiological state of fish under nanoparticles stress that can threaten aquatic health.
Behrooz Heidari; Sanaz Avarjeh; Hasan Taghavi Jelodar
Volume 4, Issue 2 , December 2015, , Pages 25-35
Abstract
Environmental factors including salinity and temperature can affect the physiology and performance of the growth of fish, so many of internal organs such as gill may be showed wide range of morphological and functional changes. Fingerling common carp were exposed for 21 days in groups at 20, 25 ...
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Environmental factors including salinity and temperature can affect the physiology and performance of the growth of fish, so many of internal organs such as gill may be showed wide range of morphological and functional changes. Fingerling common carp were exposed for 21 days in groups at 20, 25 and 30°C and three salinity levels 0, 5 and 10ppt. Histological changes of gills tissue of common carp with average weight and length 38.28 ± 0.58 g and 14.04 ± 0.10 cm respectively is studied. After biometry, sampling was performed on 7 and 21 and transferred to the laboratory. The gill arc was fixed in Bouin's solution for 24-48 hours. To evaluate the chloride cells in the gill tissue of the fish, PAS-hematoxylin were used. The results showed that the size and number of chloride cells increased by increasing the temperature and salinity. The maximum number and size of chloride cells was observed at treatment with temperature 30 °C and salinity 10ppt. In conclusion, it could be stated that the number and size of chloride cells in the gill were affected by combined environmental factors, salinity and temperature.
behrooz . Heidari; Elham Abdzadeh; Fatemeh Nazarhaghighi3
Volume 2, Issue 1 , March 2014, , Pages 21-32
Abstract
To evaluate the levels of cortisol, glucose and other possible indicators of goldfish, Carassius auratus, in response to stress, in the first experiment, Bisphenol A (0.5 mg/L), Naphthalene (200 μg/L) and Butachlor (60%) (0.28 µg/L) were added to the aquariums water for two weeks. In the second ...
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To evaluate the levels of cortisol, glucose and other possible indicators of goldfish, Carassius auratus, in response to stress, in the first experiment, Bisphenol A (0.5 mg/L), Naphthalene (200 μg/L) and Butachlor (60%) (0.28 µg/L) were added to the aquariums water for two weeks. In the second experiment, intra-peritoneal injections of Bisphenol A, Naphthalene and Butachlor, with dose 50 mg/kg, 50 mg/kg, 20 μL on the body weight of fish were performed. On the fifth day and the end of the experiment (fifteenth day) as well as 48 hours after injection, blood sampling from caudal vein was taken and levels of cortisol, glucose, total protein and inorganic phosphorous in blood plasma were measured. The results showed that the cortisol and glucose levels on day 5, was a significant difference between control and Butachlor treatments (P <0.05) and this difference was not observed in the other treatments. In addition, there were not statistically significant differences in the plasma levels of cortisol, glucose, total protein and inorganic phosphorus in fish under stress and control samples at the end of the experiment (fifteenth day) and injected samples (P>0.05). Comparing the results of the analysis of blood plasma in the fifth and fifteenth days of experiment, it was found that levels of cortisol and glucose in the treatment Butachlor had significantly different together within two periods (P<0.05). In general, it seems that the goldfish can be resistant to aquatic pollutants and respond time to the environmental stresses is between 3 to 5 days.
